Catalog Number | GTX14224 |
Product Name | alpha 1 Fetoprotein antibody |
Product Description | Chicken polyclonal to alpha 1 Fetoprotein |
Synonyms | 174 | AFP | 104150 | P02771 | FETA | HPAFP | |
Background | Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. |
Clonality | Polyclonal |
Host | Chicken |
Isotype | IgY |
Immunogen | Synthetic peptide: LWAARYDKII PSCCKAENAV ECFQTKAATV TKELRESSLL NQHACAVMKN FGTRTFQAIT VTKLSQKFTK VNFTEIQKLV LDVAHVHEHC CRGDVLDCLQ DGEKIMSYIC SQQDTLSNKI TECCKLTTLE RGQCIIHAEN DEKPEGLSPN LNRFLGDRDF NQFSSGEKNI FLASFVHEYS RRHPQLAVSV ILRVAKGYQE LLEKCFQTEN PLECQDKGEE ELQKYIQESQ ALAKRSCGLF , corresponding to amino acids 180-420 of Human alpha 1 Fetoprotein. |
Antigen Species | Human |
Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For WB: Use at an assay dependent dilution. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end researcher. |
Species Cross Reactivity | Human - Not tested in other species |
Target | alpha 1 Fetoprotein |
Cellular Localization | Secreted |
Form Supplied | Liquid |
Purification | Affinity purified with antigen |
Storage Buffer | PBS, no preservatives added |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
Image:img24649.jpgWestern blot analysis of alpha 1 Fetoprotein onE coli-derived fusion protein as test antigen, using GTX14224at a 1:2,000 dilution. Print:Print