| Catalog Number | GTX23969 |
| Product Name | alpha 1 Fetoprotein [AFP-11] antibody |
| Product Description | Mouse monoclonal [AFP - 11] to alpha 1 Fetoprotein |
| Synonyms | 174 | AFP | 104150 | P02771 | FETA | HPAFP | |
| Background | Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. |
| Clonality | Monoclonal |
| Clone No | AFP-11 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Full length native protein (purified) (Human). |
| Antigen Species | Human |
| Tested Applications | ELISA, IHC (Formalin-fixed paraffin-embedded sections), Radioimmunoassay. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA: Use at an assay dependant concentration. This antibody can be used in a sandwich ELISA with GTX23980. For IHC-P: Use at an assay dependant concentration. For RIA: Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Target | alpha 1 Fetoprotein |
| Cellular Localization | Secreted |
| Form Supplied | Liquid |
| Purification | >95% by SDS-PAGE |
| Purification Note | Purified from hybridoma culture supernatant by protein-A affinity chromatography |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
