| Catalog Number | GTX14126 |
| Product Name | 14-3-3 phospho S [3i2] antibody |
| Product Description | Mouse monoclonal [3i2] to 14 - 3 - 3 Binding Motif ( phospho S ) |
| Background | This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery. |
| Clonality | Monoclonal |
| Clone No | 3i2 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled). |
| Tested Applications | ELISA, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at a dilution of 1/1000. IP: Use at a dilution of 1/20 on denatured proteins. WB: Use at a dilution of 1/4000. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | GTX14126 binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA the antibody recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences. |
| Species Cross Reactivity | Recognises the phospho 14-3-3 binding motif. |
| Target | 14-3-3 binding motif |
| Cellular Localization | Cytoplasmic |
| Purification | Protein A purified |
| Storage Buffer | Preservative: 0.02% sodium azide; Constituents: 50% Glycerol, BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5 |
