| Catalog Number | GTX19072 |
| Product Name | 58K Golgi protein antibody |
| Product Description | Goat polyclonal to 58K Golgi protein |
| Synonyms | 10841 | FTCD | 229100 | O95954 | LCHC1 | |
| Background | 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Immunogen | Synthetic peptide: SQLVECVPNFSEGKNQ, corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein. |
| Antigen Species | Human |
| Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: 1/64000. WB: Use at a concentration of 0.03 - 0.5 μg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Tested: Human.Expected to cross-react with Human and Mouse due to sequence homology. Not yet tested in other species. |
| Positive Controls | Human Liver lysate. |
| Target | 58K Golgi protein |
| Cellular Localization | Cytoplasmic and Golgi Apparatus |
| Purification | Immunogen affinity purified |
| Purification Note | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Storage Buffer | Preservative: 0.02% Sodium Azide. Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
