Catalog Number | GTX25820 |
Product Name | 58K Golgi protein antibody |
Product Description | Rabbit polyclonal to 58K Golgi protein - Golgi Marker |
Synonyms | 10841 | FTCD | 229100 | O95954 | LCHC1 | |
Background | 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Synthetic peptide: C-LREQGRGKDQPGRLK conjugated to KLH, corresponding to amino acids 203-217 of Human Golgi marker 58K protein. |
Antigen Species | Human |
Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | WB: Use at a dilution of 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. We are currently arranging to have this antibody tested in immunofluoresence. |
Specificity | This antibody recognises a 58kD band in human liver lysate. The band is not seen when using the immunising peptide for blocking. |
Species Cross Reactivity | Cross-reacts with Human and Mouse.However, the antibody does not react as strongly with the Mouse as with the Human protein. Expected to cross-react with Rat (100% identity with immunogen) and Pig (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
Target | 58K Golgi protein |
Form Supplied | Liquid |
Purification | Immunogen affinity purified |
Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: 1% BSA, PBS. pH 7.4 |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |