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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-25-386048 |
GTX26284 |
58K Golgi protein [58K-9] antibody//ReactswithRat,Human(seereview),Dog,Hamster. Predic/Mouse |
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Subject
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58K Golgi protein [58K-9] antibody |
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Description
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/ ReactswithRat,Human(seereview),Dog,Hamster. Predic/Mouse |
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Clonality
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Mono |
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Company
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GeneTex |
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Application
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FuncS, ICC, IF, IHC-P, IM, Western Blot |
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Conjugation
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Immunogen
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Contents
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| Catalog Number | GTX26284 | | Product Name | 58K Golgi protein [58K-9] antibody | | Product Description | Mouse monoclonal [58K - 9] to 58K Golgi protein - Golgi Marker | | Synonyms | 89833 | Ftcd | | | Background | 58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. | | Clonality | Monoclonal | | Clone No | 58K-9 | | Host | Mouse | | Isotype | IgG1 | | Immunogen | Full length native protein (purified) (Rat liver). | | Antigen Species | Rat | | Tested Applications | Functional studies, Immunocytochemistry, Immunofluorescence, IHC (Formalin-fixed paraffin-embedded sections), Immunomicroscopy, Western blot. The usefulness of this product in other applications has not been determined. | | Application Note | FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available. IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. | | Specificity | The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. | | Species Cross Reactivity | Reacts with Rat, Human (see review), Dog and Hamster. Predicted to react with Cow and Pig due to sequence homology. Not yet tested in other species. | | Positive Controls | For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extractAntigen MW: 58 kDa | | Target | 58K Golgi protein | | Cellular Localization | Cytoplasmic and Golgi Apparatu | | Form Supplied | Liquid | | Purification | Ascites | | Storage Buffer | Ascitic fluid with 15mM sodium azide | | Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. | Image:4662_ab6284_2.jpgIF staining was performed on COS7 cells using a 1:100 dilution of the GTX26284 primary antibody followed by hybridisation with a 1:100 dilution of the secondary anti-mouse antibody R270 with red fluorescence. Picture from review by Karin Birkenkamp-Demtroeder submitted 20 July 2004.img4662.jpgHeLa cells fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS. Stained with anti-58K Golgi protein monoclonal antibody (1:50)followed by anti-Mouse IgG conjugated to Cy3 (1:100). This picture was kindly supplied as part of the review submiPrint:Print |
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