| Catalog Number | GTX30500 |
| Product Name | 6X His antibody |
| Product Description | Goat polyclonal to 6-His |
| Synonyms | 6X His epitope tag HHHHHH epitope tag HHHHHH tag |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Immunogen | Goats were immunized with 6-His (HHHHHH) peptide. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Antigen Species | Goat |
| Tested Applications | ELISA, Immunocytochemistry, Immunohistochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA: (Coating) Use at a dilution of 1:100-1:500. (Primary) Use at a dilution of 1:1,000 - 1:30,000. For WB: (colorimetric) Use at a dilution of 1:1,000-1:10,000. (ECL) Use at a dilution of 1:1,000-1:30,000. For IHC and ICC: Use at a dilution of 1:200-1:2,000. For IP: Use at a concentration of 15-25 ul of gel slurry per 0.1-1mg lysate. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Specificity | Antibody recognizes 6-His (HHHHHH). |
| Species Cross Reactivity | Not tested in other species |
| Target | 6X His |
| Form Supplied | Liquid |
| Purification | Affinity purified with antigen |
| Storage Buffer | PBS, pH 7.2, and 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
