| Catalog Number | GTX30501 |
| Product Name | 6X His antibody |
| Product Description | Rabbit polyclonal to 6-His |
| Synonyms | 6 His antibody, 6 His epitope tag antibody. HHHHHH epitope tag antibody, HHHHHH tag antibody, His tag antibody, polyhistidine tag antibody |
| Background | Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Rabbits were immunized with 6-His (HHHHHH) peptide. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG |
| Antigen Species | Rabbit |
| Tested Applications | ELISA, Immunocytochemistry, Immunohistochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA (Coating): Use at a dilution of 1:100 - 1: 500. For ELISA (Primary): Use at a dilution of 1:1,000 - 1:30,000. For WB (colorimetric): Use at a dilution of 1:1,000. For WB (ECL): Use at a dilution of 1:1,000 - 1:30,000. For ICC: Use at a dilution of 1:100 - 1:400. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Specificity | Antibody recognizes 6-His (HHHHHH). |
| Target | 6X His |
| Form Supplied | Liquid |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
