| Catalog Number | GTX15149 |
| Product Name | 6X His tag [3H2201] antibody |
| Product Description | Mouse monoclonal [3H2201] to 6X His tag |
| Background | GTX15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | Monoclonal |
| Clone No | 3H2201 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | PAX6 transcription factor linked to 6-histidine tag |
| Tested Applications | ELISA, IHC (Frozen sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:250-1:500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user |
| Specificity | Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. In Western Blot of bacterial extracts, the antibody does not crossreact with any endogenous products. |
| Species Cross Reactivity | All His tagged proteins so far tested - Some crossreactivity with extracts of insect or mammalian cells |
| Target | 6X His tag |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified |
| Purification Note | From hybridoma culture supernatant |
| Storage Buffer | PBS, pH7.2 containing 0.1% sodium azide and 40% glycerol |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
