| Catalog Number | GTX19863 |
| Product Name | 6X His tag [SPM368] antibody |
| Product Description | Mouse monoclonal [SPM368] to 6X His tag |
| Synonyms | 6 His | 6 His epitope tag | HHHHHH epitope tag | HHHHHH tag | His tag |
| Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Clonality | Monoclonal |
| Clone No | SPM368 |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | His tagged recombinant protein. |
| Tested Applications | Affinity purification, Immunofluorescence. The usefulness of this product in other applications has not been determined. |
| Application Note | AP: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | All species |
| Positive Controls | Hexa histidine fusion protein. |
| Target | 6X His tag |
| Cellular Localization | Depends upon the localization of the parent protein tagged with hexa histidine. |
| Purification | Protein A purified |
| Purification Note | Purified from ascites fluid. |
| Storage Buffer | Preservative: Sodium Azide. Constituents: BSA, 10mM PBS. pH 7.4 |
| Storage Instruction | Store at 4ºC |
