| Catalog Number | GTX21206 |
| Product Name | 6X His tag¨ antibody (FITC) |
| Product Description | Rabbit polyclonal to 6X His tag (FITC) |
| Synonyms | 6X His epitope tag HHHHHH epitope tag HHHHHH tag |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH. |
| Antigen Species | N/A |
| Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA: Use at a dilution of 1:2,000-1:10,000. For WB: Use at a dilution of 1:1,000-1:5,000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Target | 6X His tag¨ |
| Conjugation | FITC |
| Conjuation Note | The molar FITC / protein ratio is 3.7:1 |
| Form Supplied | Liquid |
| Concentration | 11 mg/ml |
| Purification | Immunogen affinity purified |
| Purification Note | Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
