| Catalog Number | GTX23553 |
| Product Name | 6X His tag? antibody (HRP) |
| Product Description | Chicken polyclonal to 6X His tag ( HRP ) |
| Synonyms | 6X His epitope tag HHHHHH epitope tag HHHHHH tag |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | Polyclonal |
| Host | Chicken |
| Isotype | IgY |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH. |
| Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification. Western Blot: use at dilution of 1/1000. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. |
| Target | 6X His tag? |
| Conjugation | HRP |
| Conjuation Note | Conjugated to horseradish peroxidase (HRP). |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Storage Buffer | 50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
