| Catalog Number | GTX29107 |
| Product Name | 6X His tag? antibody |
| Product Description | Chicken polyclonal to 6X His tag |
| Synonyms | 6X His epitope tag HHHHHH epitope tag HHHHHH tag |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | Polyclonal |
| Host | Chicken |
| Isotype | unknown |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH. |
| Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | This antibody was an effective at 1:1000 (1 μg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
| Specificity | Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run. |
| Target | 6X His tag? |
| Form Supplied | Liquid |
| Purification | IgY fraction |
| Purification Note | Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. |
| Storage Buffer | PBS, pH 7.2, sodium azide, 0.1% |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
