| Catalog Number | GTX30505 |
| Product Name | 6X His antibody (FITC) |
| Product Description | Goat polyclonal Anti 6-His ( FITC) |
| Synonyms | 6 His antibody, 6 His epitope tag antibody. HHHHHH epitope tag antibody, HHHHHH tag antibody, His tag antibody, polyhistidine tag antibody 6-His |
| Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Clonality | Polyclonal |
| Host | Goat |
| Immunogen | Goats were immunized with 6-Histidine (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to fluorescein isothiocyanate (FITC). |
| Tested Applications | Immunocytochemistry. The usefulness of this product in other applications has not been determined. |
| Application Note | For ICC: Use at 0.5-5μg/ml. F/P ratio is 3 to 7. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Antibody recognizes 6-His (HHHHHH). |
| Target | 6X His |
| Conjugation | FITC |
| Form Supplied | Liquid |
| Purification | Affinity purified |
| Storage Buffer | PBS (pH 7.2) with 0.1% sodium azide and 0.2% BSA |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
