Catalog Number | GTX47025 |
Product Name | 6X His tag? antibody (Biotin) |
Product Description | Rabbit polyclonal to 6X His tag (Biotin) |
Synonyms | 6 His epitope tag antibody HHHHHH epitope tag antibody HHHHHH tag antibody His tag antibody |
Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.HIS-TAGR is a trademark of EMD Biosciences, Inc. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | HHHHHH conjugated with KLH |
Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For ELIS: Use at a dilution of 1:2,000 - 1:10,000. For WB: Use at a dilution of 1:1,000 - 1:5,000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
Specificity | This antibody recognises 6x His tag |
Target | 6X His tag? |
Conjugation | Biotin |
Form Supplied | Liquid |
Purification | Immunogen affinity purified |
Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.01% thimerosal |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. Do not freeze. |