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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-25-386065 |
GTX77352 |
6X His antibody//Nottestedinotherspecies/Rabbit |
1/EA |
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문의 |
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Subject
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6X His antibody |
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Description
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/ Nottestedinotherspecies/Rabbit |
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Clonality
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Poly |
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Company
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GeneTex |
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Application
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Western Blot |
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Conjugation
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Immunogen
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Contents
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| Catalog Number | GTX77352 | | Product Name | 6X His antibody | | Product Description | Affinity Purified Rabbit anti - 6xHIS | | Clonality | Polyclonal | | Host | Rabbit | | Isotype | IgG | | Immunogen | Rabbits were immunized with HHHHHH conjugated with KLH. After multiple immunizations in Freunds adjuvant serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support | | Antigen Species | Rabbit | | Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. | | Target | 6X His | | Form Supplied | Liquid | | Storage Buffer | Phosphate Buffered Saline, pH 7.2, 0.1% sodium azide | | Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. | | Notes | WB: 293T cells were transfected with three different constructs of differing sizes. Whole cell extracts were analyzed and the results compared with lysates from non-transfected cells. The anti 6HIS was an effective antibody at 1:1000 ( 1 ug/ml) dilution (probably can use even more dilute) using ICL, Inc affinity purified goat anti rabbit IgG HRPO at 1:5000. Also, using another positive control (Invitrogen Positope R900-50), a 1:10,000 dilution of the anti 6HIS gave a band against 100 ng and 25 ng of the Positope using a colorimetric detection system. For Research and Manufacturing Only; Not for Use in Diagnostic Applications. ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA(HHHHHH-BSA). A 1:50,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using ICL, Inc HRPO conjugated Goat Anti Rabbit IgG at 1:28,000 and TMB as the substrate. Appropriate specificity controls were run" | |
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