| Catalog Number | GTX77354 |
| Product Name | 6X His antibody (HRP) |
| Product Description | Rabbit polyclonal to 6-His (HRP) |
| Synonyms | 6X His 6-His 6His 6xHIS |
| Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Rabbits were immunized with HHHHHH conjugated with KLH |
| Antigen Species | Rabbit |
| Tested Applications | ELISA, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For WB: Use at a dilution of 1:1,000-1:5,000. For ELISA: Use at a dilution of 1:5,000-1:20,000. For ICC: Use at a dilution of 1:200-1:500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Target | 6X His |
| Conjugation | HRP |
| Conjuation Note | The affinity purified antibodies were conjugated with horseradish peroxidase (HRP) |
| Form Supplied | Liquid |
| Purification | Affinity purified with antigen |
| Purification Note | From polyclonal serum |
| Storage Buffer | 50 mM HEPES pH7.0, 0.1 M Sodium Chloride containing 0.2% BSA and 0.01% Thimerosal |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
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