| Catalog Number | GTX11734 |
| Product Name | ACE [2E2] antibody |
| Product Description | Mouse monoclonal [2E2] to Angiotensin Converting Enzyme |
| Synonyms | 1636 | ACE | 104300 | P12821 | ACE1 | CD143 | DCP | DCP1 | MGC26566 | |
| Background | ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. |
| Clonality | Monoclonal |
| Clone No | 2E2 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Denatured Human kidney Angiotensin Converting Enzyme. |
| Antigen Species | Human |
| Tested Applications | ELISA, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of neat - 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: Use at a dilution of 1/50 - 1/100. Predicted molecular weight: 170 (somatic) or 90 (germinal) kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | GTX11734 recognises human Angiotensin Converting Enzyme. |
| Species Cross Reactivity | Cross-reacts with Human, Mouse, Rat, Cow, Cat, Dog, Guinea pig, Monkey and Rabbit. Not yet tested in other species. |
| Positive Controls | For immunohistology use kidney tissue (lung in rat studies) |
| Target | ACE |
| Cellular Localization | Type I membrane protein |
| Purification | Tissue culture supernatant |
| Storage Buffer | Preservative: 0.09% Sodium Azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | This product should be stored undiluted.Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. |
