| Catalog Number | GTX11738 |
| Product Name | ACE [9B9] antibody |
| Product Description | Mouse monoclonal [ 9B9] to Angiotensin Converting Enzyme |
| Synonyms | 1636 | ACE | 104300 | P12821 | ACE1 | CD143 | DCP | DCP1 | MGC26566 | |
| Background | ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. |
| Clonality | Monoclonal |
| Clone No | 9B9 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Human lung Angiotensin converting enzyme. |
| Antigen Species | Human |
| Tested Applications | ELISA, Flow cytometry/FACS, IHC (Frozen sections), Immunoprecipitation. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at an assay dependent dilution. FACS: Use at a dilution of neat - 1/10. Use 10ul of the suggested working dilution to label 1E6 cells in 100ul. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Is unsuitable for IHC-P or WB. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | GTX11738 recognises human somatic Angiotensin Converting Enzyme.This antibody recognises active ACE binding to an N-terminal domain epitope, different to that recognised by clone i2H5 (GTX11735, GTX11736). |
| Species Cross Reactivity | Cross-reacts with Human, Rat, Cat, Hamster and Monkey. Not yet tested in other species. |
| Positive Controls | kidney |
| Target | ACE |
| Cellular Localization | Type I membrane protein |
| Purification | Tissue culture supernatant |
| Storage Buffer | Preservative: 0.09% Sodium Azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | This product should be stored undiluted.Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. |
