| Catalog Number | GTX22803 |
| Product Name | ACHE [HR2] antibody |
| Product Description | Mouse monoclonal [HR2] to Acetylcholinesterase |
| Synonyms | 43 | ACHE | 100740 | P22303 | YT | |
| Background | Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. Ache is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft. |
| Clonality | Monoclonal |
| Clone No | HR2 |
| Host | Mouse |
| Isotype | IgG2b |
| Immunogen | Purified human cerebellar acetylcholinesterase |
| Antigen Species | Human |
| Tested Applications | ELISA, IHC (Frozen sections), Immunoprecipitation. The usefulness of this product in other applications has not been determined. |
| Application Note | Immunohistochemistry: use at a dilution of 1/50. ELISA: Assay dependent. Immunoprecipitation: Assay dependent. Not tested in any other applications. Optimal dilutions / concentrations should be determined by the end user. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. |
| Specificity | This antibody does not detect butyrylcholinesterase (BChE). |
| Species Cross Reactivity | Cross-reacts with Human, Rabbit, Cow, Cat and Guinea pig. Not yet tested in other species. |
| Target | ACHE |
| Form Supplied | Liquid |
| Purification | Ascites |
| Storage Buffer | PBS with 0.05% sodium azide |
| Storage Instruction | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
