| Catalog Number | GTX21900 |
| Product Name | Acrosin [ACR-2] antibody |
| Product Description | Mouse monoclonal [ACR - 2] to Acrosin |
| Synonyms | ACR Acrosin precursor ACRS Proacrosin |
| Background | Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. |
| Clonality | Monoclonal |
| Clone No | ACR-2 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization. |
| Tested Applications | Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ICC: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Specificity | The antibody ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa. |
| Species Cross Reactivity | Porcine - Does not cross-react with Human, Bovine, and Dog - Not yet tested in other species |
| Target | Acrosin |
| Form Supplied | Liquid |
| Purification | Protein A purified |
| Purification Note | >95% (by SDS-PAGE) |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15mM sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
