| Catalog Number | GTX76100 |
| Product Name | F-actin [NH3] antibody |
| Product Description | Mouse monoclonal [NH3] to human actin sample |
| Background | Filamentous (F) actin is a principle cytoskeletal element in some non-muscle cells including polymorphonuclear leukocytes (PMNs). |
| Clonality | Monoclonal |
| Clone No | NH3 |
| Host | Mouse |
| Isotype | IgM |
| Immunogen | Human monocytes and U937 cell line |
| Antigen Species | Human |
| Tested Applications | Flow cytometry/FACS, IHC (Frozen sections). The usefulness of this product in other applications has not been determined. |
| Application Note | For Flow cytometry: Use at a dilution of 1:5 - 1:25. For IHC-Fr: Use at an assay dependent dilution. Not tested in other applications. Optimal concentrations/dilutions should be determined by the researcher. |
| Specificity | GTX76100 recognises human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. |
| Species Cross Reactivity | Rabbit - Not yet tested in other species |
| Target | F-actin |
| Form Supplied | Liquid |
| Purification | Purified IgM |
| Purification Note | From hybridoma culture supernatant |
| Storage Buffer | Phosphate buffered saline pH 7.4, containing 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
