| Catalog Number | GTX17146 |
| Product Name | Actin [1A4+5C5] antibody |
| Product Description | Mouse monoclonal [1A4+5C5] to Actin |
| Background | Useful in identifying tumors arising from muscle cells, i.e. leiomyosarcoma as well as rhabdomyosarcoma. |
| Clonality | Monoclonal |
| Clone No | 1A4+5C5 |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5. |
| Antigen Species | Mouse |
| Tested Applications | IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IHC-P: 1/25 - 1/50. An incubation period of 30 minutes at room temperature usingf an ABC method is recommended. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Human, Rat, Rabbit, Cow, Guinea pig, Sheep and Snake. Not yet tested in other species. |
| Positive Controls | Skeletal muscle |
| Target | Actin |
| Cellular Localization | Cytoplasmic |
| Purification | Ascites |
| Storage Buffer | Preservative: Sodium Azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
