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| Cat. No |
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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-25-386106 |
GTX11004 |
Actin [AC-40] antibody (Cy3)//Cross/Mouse |
1/EA |
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Subject
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Actin [AC-40] antibody (Cy3) |
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Description
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/ Cross/Mouse |
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Clonality
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Mono |
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Company
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GeneTex |
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Application
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IF, IHC-Fr, IHC-P |
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Conjugation
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Immunogen
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Contents
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| Catalog Number | GTX11004 | | Product Name | Actin [AC-40] antibody (Cy3) | | Product Description | Mouse monoclonal [AC - 40] to Actin ( Cy3 ) | | Synonyms | 58 | ACTA1 | 102610 | P68133 | ACTA | ASMA | NEM1 | NEM2 | NEM3 | | | Background | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. | | Clonality | Monoclonal | | Clone No | AC-40 | | Host | Mouse | | Isotype | IgG2a | | Immunogen | Synthetic peptide: SGPSIVHRKCF (human) attached to a Multiple Antigen Peptide (MAP) backbone. | | Antigen Species | Human | | Tested Applications | Immunofluorescence, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. | | Application Note | IF: Use at a dilution of 1/50 (determined by direct immunofluorescent labeling of cultured human or chicken fibroblasts). The conjugate is suitable for dual immunofluorescent staining procedures. May be used for the localization of actin using direct immunofluorescence on cultured cells and frozen or methanol-fixed tissue sections. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Direct Immunofluorescent Labeling of Cultured Cells:Materials:1. Coverslips.2. Cells (e.g., human or chicken fibroblasts) in DMEM medium + 10% Fetal Calf Serum 3. 10 mM phosphate buffered saline (PBS), pH 7.2-7.4, without preservative 4. PBS containing 1% BSA (diluent).5. Absolute methanol cooled to ?20 °C.6. Acetone, Analytical grade, cooled to ?20 °C (1:1 v/v).7. Aqueous mounting medium.8. Cy3 Conjugated Monoclonal anti-ActinCell Growth and Fixation:1. Collect cells from tissue culture dish at a stage of almost confluency, wash with medium and seed on to coverslips. Seed 1-2 x 10^4 cells per cover slip and grow cells in incubator for 2-3 days. Do not change medium.2. Remove coverslips from incubator, discard medium.3. Wash twice with PBS, remove solution by aspiration.4. Add enough cold methanol to cover the cell layer. Incubate 10 minutes at ?20 °C. Aspirate solution.5. Cover cell layer with cold acetone. Incubate 1 minute at ?20 °C. Aspirate solution.6. Wash twice with PBS. Rehydrate in PBS for at least 30 minutes prior to labeling with antibody. Test:1. Dilute Monoclonal anti-Actin Cy3 Conjugate 1:50 in PBS containing 1% BSA (diluent). Add enough diluted antibody to cover the cell layer and incubate coverslip for 60 minutes at room temperature.2. Wash 3 times with PBS, 5 minutes each.3. Drain excess solution by touching edge of coverslip on paper toweling.4. Invert onto mounting medium applied on glass slides.5. Read under the UV fluorescence microscope. Mounted preparations can be stored in the dark at 2-8 °C. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. | | Specificity | This antibody does not react with the epitope located on the N-terminus of Actin. | | Species Cross Reactivity | Cross-reacts with Human, Mouse, Rat, Xenopus, Cow, Carp, Chicken, Dog, Goat, Guinea pig, Hamster, Pig, Rabbit, Sheep, Snail and Viper. Not yet tested in other species. | | Target | Actin | | Cellular Localization | Cytoplasmic | | Conjugation | Cy3 | | Form Supplied | Liquid | | Purification | Protein A purified | | Purification Note | The antiboy is Protein A purified prior to conjugation. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. | | Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 | | Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. | | Notes | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use." | |
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