| Catalog Number | GTX11006 |
| Product Name | Actin [KJ43A] antibody |
| Product Description | Mouse monoclonal [KJ43A] to Actin |
| Synonyms | 58 | ACTA1 | 102610 | P68133 | ACTA | ASMA | NEM1 | NEM2 | NEM3 | |
| Background | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Clonality | Monoclonal |
| Clone No | KJ43A |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Full length native cytosolic actin (purified) (A. proteus). |
| Tested Applications | Double immunodiffusion, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | DID: Use at an assay dependent dilution. WB: Use at a dilution of 1/100. The working dilution was determined by immunoblotting using a chicken gizzard extract and immunoperoxidase staining procedures. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | In immunoblotting assays this antibody is found to react with an actin epitope present in gizzard, skeletal and cardiac muscle. |
| Species Cross Reactivity | Cross-reacts with Acanthamoeba castellani, Human erythrocytes,Cow, Pig, Rabbit and Xenopus. Not yet tested in other species. |
| Target | Actin |
| Cellular Localization | Cytoplasmic |
| Purification | Ascites |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
| Storage Instruction | Store at -20°C. |
| Notes | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use." |
