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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-25-386109 |
GTX14128 |
Actin [2Q1055] antibody//Cross/Mouse |
1/EA |
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문의 |
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Subject
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Actin [2Q1055] antibody |
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Description
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/ Cross/Mouse |
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Clonality
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Mono |
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Company
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GeneTex |
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Application
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ELISA, ICC, IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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| Catalog Number | GTX14128 | | Product Name | Actin [2Q1055] antibody | | Product Description | Mouse monoclonal [2Q1055] to Actin | | Background | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. | | Clonality | Monoclonal | | Clone No | 2Q1055 | | Host | Mouse | | Isotype | IgG1 | | Immunogen | Chicken gizzard actin | | Antigen Species | Chicken | | Tested Applications | ELISA, Immunocytochemistry, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. | | Application Note | ELISA: Use at an assay dependent dilution. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. ICC: Use at a concentration of 10 μg/ml on methanol fixed mouse 3T3 cells. IHC-P: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 1 - 20 μg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Predicted molecular weight: 43 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. | | Specificity | GTX14128 recongizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms, it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. GTX14128 labels myotubes and stains myoblasts and fibroblasts, it does not interfer with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, GTX14128 shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. | | Species Cross Reactivity | Cross-reacts with a wide range of species. | | Target | Actin | | Cellular Localization | Cytoplasmic | | Form Supplied | Liquid | | Purification | Protein A purified | | Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 40% Glycerol, 10mM PBS. pH 7.6 | | Storage Instruction | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. | | Notes | For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer." | |
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