| Catalog Number | GTX14129 |
| Product Name | Actin [3G1] antibody |
| Product Description | Mouse monoclonal [3G1] to Actin |
| Synonyms | 25365 | Actg2 | ACTGE | MGC105507 | SMGA | |
| Background | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Clonality | Monoclonal |
| Clone No | 3G1 |
| Host | Mouse |
| Isotype | IgM |
| Immunogen | Rabbit muscle |
| Antigen Species | Rabbit |
| Tested Applications | IHC (Frozen sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | IHC-Fr: Use at a dilution of 1/5 - 1/25. Stains actin in eukaryotic cells including beta, gamma and delta. It can be used on frozen sections for the differentiation of benign (positive staining) and malignant (usually negative) papillary lesion of the breast. WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | GTX14129 recognizes rabbit alpha actin and is reactive with human actin. GTX14129 is crossreactive with actin in eukaryotic cells including beta, gamma and delta. No reactivity is observed with other cytoskeletal components. |
| Species Cross Reactivity | Cross-reacts with Human and Rabbit. Not yet tested in other species. |
| Target | Actin |
| Cellular Localization | Cytoplasmic |
| Purification | Ascites |
| Storage Buffer | Preservative: 0.01% Thimerosal / MerthiolateConstituents: Raw Ascites |
| Notes | For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer." |
