| Catalog Number | GTX17797 |
| Product Name | Actin [SPM160] antibody |
| Product Description | Mouse monoclonal [SPM160] to Actin |
| Synonyms | 58 | ACTA1 | 102610 | P68133 | ACTA | ASMA | NEM1 | NEM2 | NEM3 | |
| Background | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Clonality | Monoclonal |
| Clone No | SPM160 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Full length native protein (purified). |
| Tested Applications | Immunofluorescence, IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IF: Use at an assay dependent dilution. IHC-P: 1/50. Antigen retrieval is not essential but may optimise staining; (stain at RT for 30 minutes). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | This antibody reacts with alpha smooth muscle as well as alpha skeletal andalpha cardiac (sarcomeric) isoform of actin. It is a pan muscle actin and reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas). |
| Species Cross Reactivity | Cross-reacts with Human, Mouse, Rat, Cow, Dog, Horse and Pig. Not yet tested in other species. |
| Positive Controls | Muscle or sarcoma |
| Target | Actin |
| Cellular Localization | Cytoplasmic |
| Form Supplied | Liquid |
| Purification | Protein G purified |
| Purification Note | Purified from ascites fluid by Protein G |
| Storage Buffer | Preservative: Sodium Azide. Constituents: BSA, 10mM PBS. pH 7.4 |
| Storage Instruction | Store at 4ºC |
