| Catalog Number | GTX23232 |
| Product Name | Adenovirus Fiber trimer [2A6] antibody |
| Product Description | Mouse monoclonal [2A6] to Adenovirus Fiber trimer |
| Background | Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta sheet and beta bends, and a knob at the C terminus that contains the type specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion. |
| Clonality | Monoclonal |
| Clone No | 2A6 |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | UV irradiated Ad2 virus. |
| Tested Applications | Immunofluorescence, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For IF: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Specificity | Recognizes only trimers (high molecular weight bands 180 - 200kDa) on Western blots of unboiled Ad2 and Ad5 fiber proteins and shows immunofluorescence with Ad5 fiber mutant H5ts142 infected cells at 32ºC but not at 39.5ºC. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | Adenovirus infected cells and tissues. |
| Target | Adenovirus Fiber trimer |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Purification | Protein A affinity purified |
| Storage Buffer | 10mM Phosphate-buffered saline, pH7.4 containing 0.2% BSA and 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
