| Catalog Number | GTX16086 |
| Product Name | Acrp30 [Ne.Na] antibody |
| Product Description | Mouse monoclonal [Ne.Na] to Acrp30 |
| Synonyms | 9370 | ADIPOQ | 605441 | Q15848 | ACDC | ACRP30 | APM-1 | APM1 | GBP28 | adiponectin | |
| Background | Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase, leptin, and adipocyte complement related protein of 30 kDa (Acrp30). Acrp30 is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Acrp30 have been shown to be induced over 100-fold. Studies indicate that Acrp30 enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Acrp30 function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Acrp30 secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Acrp30 have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNF alpha suggests that Acrp30 may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Acrp30 is down-regulated in various forms of obesity and its structural similarity to TNF alpha, it is currently being investigated as an important regulator of whole body energy homeostasis. |
| Clonality | Monoclonal |
| Clone No | Ne.Na |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Recombinant human ACRP30 headless (tail adiponectin) protein. |
| Antigen Species | Human |
| Tested Applications | ELISA, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 29 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognizes human ACRP30headless (tail of adiponectin). Does not cross-react with full length adiponectin. |
| Species Cross Reactivity | Human - Does not cross-react with Mouse - Not yet tested in other species |
| Target | Acrp30 |
| Cellular Localization | Secreted |
| Form Supplied | Liquid |
| Purification | >95% by SDS-PAGE |
| Storage Buffer | Phosphate-buffered saline containing 0.02% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
