| Catalog Number | GTX23777 |
| Product Name | Aggrecan xxPEN [BC-4] antibody |
| Product Description | Mouse monoclonal [BC - 4] to Aggrecan xxPEN |
| Synonyms | 176 AGC 1 AGC1 AGC1 protein Aggrecan 1 Aggrecan core protein Aggrecan1 Cartilage specific Chondroitin sulfate proteoglycan 1 CSPCP CSPG 1 CSPG1 CSPGCP Large aggregating MSK16 SEDK Spondyloepiphyseal dysplasia Kimberley type |
| Background | This metabolite usually remains in the tissue complexed to hyaluronan but with extensive cartilage catabolism it may be released from the tissue. |
| Clonality | Monoclonal |
| Clone No | BC-4 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | ...DIPEN synthetic peptide conjugate |
| Tested Applications | ELISA, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. For IHC-Fr: Use at an assay dependant dilution. This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. For WB: Use at a dilution of 1:100. Detects a band of approximately 60 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognises the N-terminal neoepitope sequence (...DIPEN) generated at the ?MMP cleavage site? after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.. (Human sequence enumeration). Detects a band of approximately 60 kDa. |
| Species Cross Reactivity | Human, Guinea pig, Horse, Pig and Rat - Does not cross-react with cow - Not yet tested in other species |
| Target | Aggrecan xxPEN |
| Form Supplied | Liquid |
| Concentration | 0.1 mg/ml |
| Purification | Protein G affinity purified |
| Storage Buffer | Phosphate-buffered saline, no preservative added. |
| Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Notes | Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10μg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immu |
