Catalog Number | GTX22733 |
Product Name | M6PR [2G11] antibody |
Product Description | Mouse monoclonal [2G11] to Mannose 6 Phospate Receptor - Late Endosome Marker |
Synonyms | 300 kDa mannose 6 phosphate receptor antibody Cation independent mannose 6 phosphate receptor antibody CD222 antigen antibody CI Man 6 P receptor antibody CI MPR antibody CIMPR antibody IGF 2 receptor antibody IGF 2R antibody IGF II receptor antibody IGF2 receptor antibody IGF2R antibody Insulin like growth factor 2 receptor antibody Insulin like growth factor II receptor antibody M6P R antibody M6P/IGF2 receptor antibody M6P/IGF2R antibody M6PR antibody MPR 300 antibody MPR300 antibody MPRI antibody |
Background | Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPRs is predominantly within late endosomes, fewer in the trans Golgi network and ~10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands. |
Clonality | Monoclonal |
Clone No | 2G11 |
Host | Mouse |
Isotype | IgG2a |
Immunogen | Purified bovine 300 kDa CI-MPR |
Antigen Species | Bovine |
Tested Applications | Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For IF: Use at an assay dependent dilution. For IHC: Use at a concentration of 2-5 ¥ìg/ml. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1 ¥ìg/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
Species Cross Reactivity | Human, Non-human primate, Rat, and Bovine - Not yet tested in other species |
Target | M6PR |
Form Supplied | Liquid |
Purification | IgG fraction |
Storage Buffer | Phosphate-buffered saline containing 0.05% sodium azide |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
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