| Catalog Number | GTX18184 |
| Product Name | 6X His tag [HIS.H8] antibody |
| Product Description | Mouse monoclonal [HIS.H8] to 6X His tag |
| Synonyms | 6 His | 6 His epitope tag | HHHHHH epitope tag | HHHHHH tag | His tag |
| Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Clonality | Monoclonal |
| Clone No | HIS.H8 |
| Host | Mouse |
| Isotype | IgG2b |
| Immunogen | 6x His synthetic peptide. |
| Tested Applications | Dot blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For dot blot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. For immunocytochemistry: Use at 1:200. IF: Use at 1:200. For immunoprecipitation: Use at an assay dependent dilution. For western blot: 1:1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. |
| Target | 6X His tag |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | IgG fraction |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: PBS. pH 7.4 |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
