| Catalog Number | GTX76152 |
| Product Name | 1A antigen [MRC OX-3] antibody (FITC) |
| Product Description | Mouse monoclonal [MRC OX-3] to RT1Bu (FITC) |
| Clonality | Monoclonal |
| Clone No | MRC OX-3 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Rat thymocyte membrane glycoprotein |
| Antigen Species | Rat |
| Tested Applications | Flow cytometry/FACS. The usefulness of this product in other applications has not been determined. |
| Application Note | For FACS: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. Recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras. |
| Species Cross Reactivity | Rat and Mouse - Not yet tested in other species |
| Target | 1A antigen |
| Conjugation | FITC |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified |
| Purification Note | From mouse ascitic fluid |
| Storage Buffer | PBS, pH7.2 containing BSA and 0.02% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
