| Catalog Number | GTX28767 |
| Product Name | alpha 1 Antitrypsin antibody |
| Product Description | Sheep polyclonal to alpha 1 Antitrypsin |
| Synonyms | 5265 | SERPINA1 | 107400 | P01009 | A1A | A1AT | AAT | MGC23330 | MGC9222 | PI | PI1 | PRO2275 | |
| Background | α1-antitrypsin is a plasma protein synthesized in the liver. It consists of a single polypeptide chain and has a molecular weight of 51 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of ·1-antitrypsin in plasma is1.3g/L. |
| Clonality | Polyclonal |
| Host | Sheep |
| Isotype | IgG |
| Immunogen | Human serum α1-antitrypsin purified from plasma and shown to be homogeneous by SDS-PAGE. |
| Antigen Species | Human |
| Tested Applications | Double immunodiffusion, Immunoelectrophoresis, Radial immunodiffusion, Rocket immunoelectrophoresis. The usefulness of this product in other applications has not been determined. |
| Application Note | RID and Rocket IEP: 2μL antiserum/cm= gel vs 5μL 1/2 - 1/50 dilution human serumDouble diffusion: 10μL antiserum vs 5μL serumIEP: 100μL antiserum vs 5μL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded. |
| Specificity | This product gives a single arc when tested by IEP and 2D-IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human ·1-antitrypsin. |
| Species Cross Reactivity | Cross-reacts with Human. Does not cross-react with Rabbit, Rat, Horse, Mouse, Cow, Dog, Pig, Cat, Guinea pig or Chicken. Not yet tested in other species. |
| Target | alpha 1 Antitrypsin |
| Cellular Localization | Secreted |
| Purification | IgG fraction |
| Purification Note | Antiserum is prepared by immunisation of sheep with human ·1-antitrypsin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents.An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2?m filtered. |
| Storage Buffer | Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
| Notes | Slight precipitation can occur on storage which may be removed by centrifugation and should not affectperformance characteristics. |
