| Catalog Number | GTX28770 |
| Product Name | alpha 2 Macroglobulin antibody |
| Product Description | Sheep polyclonal to alpha 2 Macroglobulin |
| Synonyms | 2 | A2M | 103950 | P01023 | CPAMD5 | DKFZp779B086 | FWP007 | S863-7 | alpha 2M | |
| Background | α2-Macroglobulin is a plasma protein, mainly synthesized in the liver. It is a tetrameric molecule, with a molecular weight of 720 kDa and usually occurs in serum at 1000-1500 mg/L. Its main function is as a protease inhibitor. Following conformational change, the protease is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites, which react with receptors on a variety of cells including macrophages and liver cells. |
| Clonality | Polyclonal |
| Host | Sheep |
| Isotype | IgG |
| Immunogen | Human α2-macroglobulin purified from plasma. |
| Antigen Species | Human |
| Tested Applications | Double immunodiffusion, Immunoelectrophoresis, Radial immunodiffusion, Rocket immunoelectrophoresis. The usefulness of this product in other applications has not been determined. |
| Application Note | RID and Rocket IEP: 1μL antiserum/cm= gel vs 5μL neat - 1/10 dilution human serumDouble diffusion: 10μL antiserum vs 5μL serumIEP: 100μL antiserum vs 5μL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded. |
| Specificity | This product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity is confirmed by double diffusion (Ouchterlony) vs human serum and a known anti-human ·2-Macroglobulin. |
| Species Cross Reactivity | Cross-reacts with Human, Rabbit, Horse, Cow, Dog, Pig, Cat and Guinea pig. Does not cross-react with Rat, Mouse or Chicken. Not yet tested in other species. |
| Target | alpha 2 Macroglobulin |
| Purification | IgG fraction |
| Purification Note | Antiserum is prepared by immunisation of sheep with human ·2-macroglobulin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents.An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2?m filtered. |
| Storage Buffer | Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
| Notes | Slight precipitation can occur on storage which may be removed by centrifugation and should not affectperformance characteristics. |
