| Catalog Number | GTX18147 |
| Product Name | Actin smooth muscle [0.N.5] antibody |
| Product Description | Mouse monoclonal [0.N.5] to alpha smooth muscle Actin |
| Synonyms | 59 | ACTA2 | 102620 | P62736 | ACTSA | |
| Background | Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells. |
| Clonality | Monoclonal |
| Clone No | 0.N.5 |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Synthetic peptide: MCEEEDSTAL, corresponding to N terminal amino acids 1-10 of alpha smooth muscle Actin. |
| Tested Applications | Immunofluorescence, IHC (Formalin fixed sections), IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 0.5-1 ?g/ml. IHC-Fr: Use at a concentration of 0.5 ?g/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial). |
| Species Cross Reactivity | Cross-reacts with Human, Mouse, Rat, Rabbit, Cow, Baboon and Chicken. Not yet tested in other species. |
| Positive Controls | Smooth muscle or leiomyosarcoma. |
| Target | Actin smooth muscle |
| Cellular Localization | Cytoplasmic |
| Form Supplied | Liquid |
| Concentration | 0.02 mg/ml |
| Purification | Protein A purified |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 0.2% BSA, PBS. pH 7.4 |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
