| Catalog Number | GTX75513 |
| Product Name | Actin smooth muscle [1A4] antibody |
| Product Description | Mouse monoclonal [1A4] to Actin - prediluted (sample) |
| Synonyms | 59 | ACTA2 | 102620 | P62736 | ACTSA | |
| Background | Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells, and also in tumours derived from these cells. Actins are involved in maintaining the structure and integrity of a cell, as well as cell motility. They are highly conserved proteins and make up the majority of the contractile apparatus. To date, six different actin isoforms have been described. |
| Clonality | Monoclonal |
| Clone No | 1A4 |
| Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Alpha-smooth muscle actin N-terminal decapeptide |
| Tested Applications | IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | For IHC-Fr and IHC-P: Use at a neat - 1:10 dilution. Not tested in other applications. Optimal concentrations/dilutions should be determined by the researcher. *This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. |
| Specificity | Recognises smooth muscle actin, being specific for the alpha-isoform |
| Species Cross Reactivity | Sheep, Pig, Rat - Not tested in other species |
| Positive Controls | Smooth muscle from vascularised tissues such as gastrointestinal tract. |
| Target | Actin smooth muscle |
| Form Supplied | Liquid |
| Purification | Unpurified |
| Purification Note | From mouse ascitic fluid |
| Storage Buffer | Phosphate buffered saline containing 1%BSA and 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
