Catalog Number | GTX112898 |
Product Name | p21 antibody |
Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
Product Description | Rabbit polyclonal antibody to p21 (cyclin-dependent kinase inhibitor 1A (p21, Cip1)) |
Synonyms | CAP20 | CDKN1 | CIP1 | MDA-6 | P21 | SDI1 | WAF1 | p21CIP1 |
Background | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of p21 |
Antigen Species | Human |
Tested Applications | Dot blot |
Application Note | Further validation in progress. |
Specificity | This antibody is specific to the Protein antigen from p21 . |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | Target recombinant protein |
Target | p21 |
Predicted Target Size(KDa) | 18.12 |
Cellular Localization | Cytoplasm | Nucleus |
Form Supplied | Liquid |
Concentration | 0.63 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |