Catalog Number | GTX27818 |
Product Name | p105 [B301(2B3)] antibody |
Product Description | Mouse monoclonal [B301( 2B3 )] to p105 |
Background | The p105 protein found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation-associated nuclear antigen that is absent in non-cycling cells in the Go phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 , and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens. |
Clonality | Monoclonal |
Clone No | B301(2B3) |
Host | Mouse |
Isotype | IgM |
Immunogen | Activated peripheral blood nuclei. |
Tested Applications | IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For IHC:Use at a dilution of 1:50-1:100. Formalin-fixed paraffin-embedded (FFPE) tissue sections require pepsin or trypsin digestion. For WB: Use at a dilution of 1:100-1:200. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Species Cross Reactivity | Human - Not yet tested in other species |
Target | p105 |
Cellular Localization | Nuclear |
Form Supplied | Liquid |
Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.02% BSA and 0.09% sodium azide |
Storage Instruction | Keep as concentrated solution, aliquot and store at 4¡ÆC. Do not freeze. |