| Catalog Number | GTX21044 |
| Product Name | p13 [BM3] antibody |
| Product Description | Mouse monoclonal [BM3] to p13 |
| Background | p13 is a specific marker for human myeloid cells. Induction studies using HL-60 °Cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13,000 Dalton protein |
| Clonality | Monoclonal |
| Clone No | BM3 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes |
| Antigen Species | Human |
| Tested Applications | Flow cytometry/FACS, IHC (Frozen sections). The usefulness of this product in other applications has not been determined. |
| Application Note | This antibody is suitable for flow cytometry and frozen section immunohistochemistry. The antibody does not stain tissue after paraffin treatment. For immunohistochemistry dilute the antibody 1:100. Researchers should determine optimal titers for other applications. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Target | p13 |
| Form Supplied | Liquid (sterile filtered) |
| Purification | Protein A purified |
| Purification Note | This protein A purified mouse monoclonal antibody reacts specifically with p13 in human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues. The antibody recognizes a 13 kDa band corresponding to p13. Cross reactivity from other sources has not been determined. |
| Storage Buffer | 0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 |
| Storage Instruction | Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for |
