| Catalog Number | GTX82904 |
| Product Name | V5 tag antibody |
| Product Description | Rabbit Polyclonal Antibody to V5 Epitope Tag |
| Background | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the epitope tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered protein, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | GTX82904 immunogen corresponds to the V5 tag (GKP IPN PLL GLD ST). |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For WB: Use at a dilution of 1:500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Target | V5 tag |
| Form Supplied | Liquid |
| Purification | Affinity purified with antigen |
| Storage Buffer | Phosphate-buffered saline containing 1 mg/ml BSA and 0.05% sodium azide |
| Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
