Catalog Number | GTX12199 |
Product Name | UBE1L antibody |
Product Description | Rabbit polyclonal to Ube1L |
Synonyms | 7318 | UBE1L | 191325 | P41226 | D8 | MGC12713 | UBE2 | |
Background | Ube1L is a member of the E1 ubiquitin-activating enzyme family. It is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia. It has been reported that Ube1L acts as the activating enzyme in the ISG15 conjugation pathway. ISG15 is a ubiquitin-like protein and its transcription is induced by interferon treatment. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Synthetic peptide: DCDPRSIHVREDGSLEIGD, corresponding to amino acids 222-240 of Human Ube1L. |
Antigen Species | Human |
Tested Applications | IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | IHC-P: Use at a dilution of 1/1000. IHC-Fr: Use at a dilution of 1/1000. WB: Use at a dilution of 1/500. Detects a band of approximately 112 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | The specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not.This antibody reacts with the N-terminus of Ube1L. |
Species Cross Reactivity | Cross-reacts with Human. Does not cross-react with Mouse. Not yet tested in other species. |
Target | UBE1L |
Form Supplied | Liquid |
Purification | Whole antiserum |
Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |