Catalog Number | GTX106854 |
Product Name | U2AF35 antibody |
Full Name | U2 small nuclear RNA auxiliary factor 1 |
Product Description | Rabbit polyclonal antibody to U2AF35 (U2 small nuclear RNA auxiliary factor 1) |
Synonyms | DKFZp313J1712 | FP793 | RN | RNU2AF1 | U2AF35 | U2AFBP |
Background | This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 23 and 237 of U2AF35 |
Antigen Species | human |
Tested Applications | WB,IHC-P,IF. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000,IHC-P: 1:100-1:250,IF:1:100-1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the Human U2AF35 protein. |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | 293T | A431 | H1299 | HeLaS3 | HepG2 | Molt-4 | Raji |
Target | U2AF35 |
Predicted Target Size(KDa) | 27.88 |
Cellular Localization | Nucleus |
Form Supplied | Liquid |
Concentration | 0.55 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX106854 WB ImageSample(30¥ìg whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)12% SDS PAGEGTX106854 diluted at 1:1000GTX106854 IHC ImageImmunohistochemical analysis of paraffin-embedded DU145 xenograft, using U2AF35(GTX106854) antibody at 1:100 dilution.Print:Print