Catalog Number | GTX100416 |
Product Name | UBE2B antibody |
Full Name | ubiquitin-conjugating enzyme E2B (RAD6 homolog) |
Product Description | Rabbit polyclonal antibody to UBE2B (ubiquitin-conjugating enzyme E2B (RAD6 homolog)) |
Synonyms | E2-17kDa | HHR6B | HR6B | RAD6B | UBC2 |
Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq] |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 122 of UBE2B |
Antigen Species | Human |
Tested Applications | WB. The usefulness of this product in other applications has not been determined. |
Application Note | Suggested starting dilutions are as follows. WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Specificity | This antibody is specific to the Human UBE2B protein. |
Species Cross Reactivity | Human - Not yet tested in other species |
Positive Controls | HeLa | HepG2 |
Target | UBE2B |
Predicted Target Size(KDa) | 17.32 |
Cellular Localization | Cell membrane (By similarity) | Nucleus (By similarity) |
Form Supplied | Liquid |
Concentration | 0.71 mg/ml |
Purification | Purified by antigen-affinity chromatography. |
Storage Buffer | 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid multiple freeze-thaw cycles. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Image:GTX100416 WB ImageSample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE UBE2B antibody GTX100416 diluted at 1:1000 Print:Print