| Catalog Number | GTX24564 |
| Product Name | 53BP1 antibody |
| Product Description | Rabbit polyclonal to 53BP1 |
| Background | p53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and re-localizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability. |
| Clonality | polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | A synthetic peptide, which represented a portion of human tumor protein p53 binding protein 1 encoded in part by exon 11 and 12. |
| Antigen Species | Human |
| Tested Applications | Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ICC (human and mouse): Use at a dilution of 1/100 - 1/500. WB (human only): Use at a dilution of 1/1000 - 1/10000 (ECL). Detects a band of approximately 220 kDa. Irradiated lysates demonstrate a gel shift. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Specific for human 53BP1 protein. |
| Species Cross Reactivity | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Positive Controls | U205 and 293T cell lysates. |
| Target | 53BP1 |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Tris-citrate/phosphate. pH 7.8 |
| Storage Instruction | Store at 4 degrees C. Avoid repeated freeze-thaw cycles. |
