| Catalog Number | GTX29136 |
| Product Name | 6-His tag antibody |
| Product Description | Goat polyclonal to 6-His |
| Synonyms | 6X His epitope tag, HHHHHH epitope tag, HHHHHH tag, |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | polyclonal |
| Host | Goat |
| Isotype | IgG |
| Immunogen | Synthetic peptide: HHHHHH (6-His) conjugated to KLH. |
| Tested Applications | ELISA, Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA (Coating): Use at a dilution of 2 - 10 ?g/ml. For ELISA (Primary): Use at a dilution of 0.03 - 1 ?g/ml. For ICC: Use at a dilution of 0.5 - 5 ?g/ml. For WB (Colorimetric): Use at a dilution of 0.1 - 1 ?g/ml. For WB (Chemiluminescent): Use at a dilution of 0.03 - 1 ?g/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | This antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA). |
| Species Cross Reactivity | Not tested in other species |
| Target | 6X His tag |
| Form Supplied | Liquid |
| Purification | Affinity purified with antigen |
| Storage Buffer | PBS, pH 7.2, and 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
