| Catalog Number | GTX29108 |
| Product Name | 6X His tag antibody |
| Product Description | Rabbit polyclonal to 6X His tag |
| Synonyms | 6X His epitope tag, HHHHHH epitope tag, HHHHHH tag, |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH |
| Tested Applications | ELISA, Flow cytometry/FACS, Immunofluorescence, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Western blotting up to 1/1000 293T cells were transfected with three different constructs of differing sizes. Whole cell extracts were analyzed and the results compared with lysates from non-transfected cells. The anti 6HIS was an effective antibody at 1:1000 ( 1 ug/ml) dilution. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding with untransfected extracts. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user |
| Specificity | ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:25,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using HRP-conjugated Goat anti-Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run. |
| Target | 6X His tag |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Storage Buffer | Phosphate-buffered saline, containing 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Notes | This antibody recognizes both C-terminal and N-terminal tags. |
