| Catalog Number | GTX21269 |
| Product Name | 6X His tag antibody |
| Product Description | Goat polyclonal to 6X His tag ( HRP ) |
| Synonyms | 6X His epitope tag, HHHHHH epitope tag, HHHHHH tag, |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | polyclonal |
| Host | Goat |
| Isotype | IgG |
| Immunogen | Synthetic peptide: HHHHHH (6-His) conjugated to KLH. |
| Tested Applications | ELISA, Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: Use at a dilution of 1/10000 - 1/100000. ICC: Use at a dilution of 1/200 - 1/500. WB: Use at a dilution of 1/1000 - 1/30000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Target | 6X His tag |
| Conjugation | HRP |
| Conjuation Note | Conjugated to horseradish peroxidase (HRP). |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Purification Note | Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. |
| Storage Buffer | 50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
