| Catalog Number | GTX21270 |
| Product Name | 6X His tag antibody |
| Product Description | Goat polyclonal to 6X His tag ( FITC ) |
| Synonyms | 6X His epitope tag, HHHHHH epitope tag, HHHHHH tag, |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | polyclonal |
| Host | Goat |
| Isotype | IgG |
| Immunogen | Synthetic peptide: HHHHHH (6-His) conjugated to KLH. |
| Tested Applications | Immunocytochemistry, Immunohistochemistry. The usefulness of this product in other applications has not been determined. |
| Application Note | ICC/IHC: Use at a dilution of 1/200 - 1/2000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Target | 6X His tag |
| Conjugation | FITC |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Purification Note | Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. |
| Storage Buffer | PBS with 0.2% BSA, 0.1% sodium azide |
| Storage Instruction | Store at 2-8 °C. Do not freeze. |
